Product Name :
Phospho-Smad3 (S423 + S425) Recombinant Rabbit Monoclonal Antibody [ST0493]
Predicted band size :
48 kDa
Observed band size :
55 kDa
Synonyms:
DKFZP586N0721 antibody DKFZp686J10186 antibody hMAD 3 antibody hMAD-3 antibody hSMAD3 antibody HSPC193 antibody HST17436 antibody JV15 2 antibody JV15-2 antibody JV152 antibody 展开
Function :
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.6-Chlorofuro[3,4-c]pyridin-1(3H)-one web Human Smad3 is subject to phosphorylation by TGFβ on specific amino acid residues, including Ser 208.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser423 and 425 of human Smad3.
Species reactivity:
Human, Mouse
Verify application:
WB, IF-Cell, IF-Tissue, IHC-P
Coupling :
unconjugated
Clone number:
ST0493
RRID:
AB_3069851
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Buy5-Bromo-6-fluorobenzo[d]thiazol-2-amine Aliquot store at -20℃ or -80℃.PMID:33487611 Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 48 kDa
Positive control :
A549 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate, C2C12 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate, HeLa starved overnight then treated with 10ng/mL TGF-β1 for 30 minutes cell lysate, NIH/3T3 starved overnight then treated with 10ng/mL TGF-β1 for 30 minutes cell lysate, A549, human large intestine tissue, mouse skin tissue, mouse kidney tissue.