Cells had been harvested, resuspended in TBSC (TBS pH 7.four, 2 mM CaCl2) with
Chemexpress Cells have been harvested, resuspended in TBSC (TBS pH 7.four, 2 mM CaCl2) with protease inhibitor mix (Comprehensive, Roche Applied Science) and lysed by sonication. Right after centrifugation, the supernatant…